ht h9523  (MedChemExpress)

Journal: International Journal of Molecular Sciences

Article Title: The Effect of the Stress Induced by Hydrogen Peroxide and Corticosterone on Tryptophan Metabolism, Using Human Neuroblastoma Cell Line (SH-SY5Y)

doi: 10.3390/ijms24054389

Figure Lengend Snippet: Summary of serotonin (5-HT)’s branch of L-tryptophan (L-Trp) biotransformation. This amino acid is ( a ) metabolized into 5-hydroxytryptophan (5-HTP), that is ( b ) converted into 5-HT, and finally ( c ) metabolized into 5-hydroxyindoleacetic acid (5-HIAA).

Article Snippet: Penicillin/streptomycin (cat. no. P4333), thiazolyl blue tetrazolium bromide (MTT, cat. no. M5655), CORT (cat. no. 27840), H 2 O 2 30% (cat. no. 1.07209), L-Trp (cat. no. T0254-5G), 5-HTP (cat. no. H9772-1G), 5-HT (cat. no. H9523-25MG), and 5-HIAA (cat. no. H8876-100MG) were acquired from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Effect of the Stress Induced by Hydrogen Peroxide and Corticosterone on Tryptophan Metabolism, Using Human Neuroblastoma Cell Line (SH-SY5Y)

doi: 10.3390/ijms24054389

Figure Lengend Snippet: Representative images (100× total magnification) of SH-SY5Y cells exposed to ( A ) 5-HTP 500 µM, ( B ) 5-HTP 500 µM + H 2 O 2 300 µM, ( C ) 5-HTP 500 µM + CORT 500 µM, and effect of 5-HTP 500 µM, 5-HTP 500 µM + H 2 O 2 300 µM, 5-HTP 500 µM + CORT 500 µM on ( D ) cellular viability and on ( E ) the variation of 5-HTP concentration, in which the baseline represents 5-HTP 500 μM. All the compounds were exposed to the cells for 48 h. Cell viability results represent the mean ± SEM of three independent assays, expressed as the percentage of the vehicle (100%). HPLC results represent the analysis of the mixture of samples from three independent experiments. Statistically significant * p < 0.05, *** p < 0.001 and **** p < 0.0001 vs. vehicle. Scale bar: 50 μm.

Article Snippet: Penicillin/streptomycin (cat. no. P4333), thiazolyl blue tetrazolium bromide (MTT, cat. no. M5655), CORT (cat. no. 27840), H 2 O 2 30% (cat. no. 1.07209), L-Trp (cat. no. T0254-5G), 5-HTP (cat. no. H9772-1G), 5-HT (cat. no. H9523-25MG), and 5-HIAA (cat. no. H8876-100MG) were acquired from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: The Effect of the Stress Induced by Hydrogen Peroxide and Corticosterone on Tryptophan Metabolism, Using Human Neuroblastoma Cell Line (SH-SY5Y)

doi: 10.3390/ijms24054389

Figure Lengend Snippet: Representative images (100× total magnification) of SH-SY5Y cells exposed to ( A ) 5-HT 500 µM, ( B ) 5-HT 500 µM + H 2 O 2 300 µM, ( C ) 5-HT 500 µM + CORT 500 µM, and effect of 5-HT 500 µM, 5-HT 500 µM + H 2 O 2 300 µM, 5-HT 500 µM + CORT 500 µM on ( D ) cellular viability, and on ( E ) the variation of 5-HT concentration, in which the baseline represents 5-HT 500 µM. All the compounds were exposed to the cells for 48 h. Cell viability results represent the mean ± SEM of three independent assays, expressed as the percentage of the vehicle (100%). HPLC results represent the analysis of the mixture of samples from three independent experiments. Statistically significant ** p < 0.01 and **** p < 0.0001 vs. vehicle. Scale bar: 50 µm.

Article Snippet: Penicillin/streptomycin (cat. no. P4333), thiazolyl blue tetrazolium bromide (MTT, cat. no. M5655), CORT (cat. no. 27840), H 2 O 2 30% (cat. no. 1.07209), L-Trp (cat. no. T0254-5G), 5-HTP (cat. no. H9772-1G), 5-HT (cat. no. H9523-25MG), and 5-HIAA (cat. no. H8876-100MG) were acquired from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: The Effect of the Stress Induced by Hydrogen Peroxide and Corticosterone on Tryptophan Metabolism, Using Human Neuroblastoma Cell Line (SH-SY5Y)

doi: 10.3390/ijms24054389

Figure Lengend Snippet: Concentrations of 5-HT (nM) in the extracellular medium of SH-SY5Y cells treated with ( A ) H 2 O 2 50–300 µM and ( B ) CORT 100–500 µM, determined by HPLC (electrochemical method). The results represent the analysis of the supernatant collected from the three independent experiments.

Article Snippet: Penicillin/streptomycin (cat. no. P4333), thiazolyl blue tetrazolium bromide (MTT, cat. no. M5655), CORT (cat. no. 27840), H 2 O 2 30% (cat. no. 1.07209), L-Trp (cat. no. T0254-5G), 5-HTP (cat. no. H9772-1G), 5-HT (cat. no. H9523-25MG), and 5-HIAA (cat. no. H8876-100MG) were acquired from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: